Eppendorf 5420 Centrifuge

• Spin-column based DNA/RNA purification.
• Gel extraction procedures.
• PCR preparation workflows.
• General molecular biology sample separation and processing.
• Micro-volume sample pelleting and clarification.

Typical capacity

Commonly accommodates 24 x 1.5/2.0 mL tubes, with adapters for smaller volumes (e.g., 0.2, 0.4, 0.5 mL) or PCR strips.

Maximum speed

Units of this type typically reach up to 15,000 rpm.

Maximum RCF

For this class of equipment, maximum RCF is commonly around 21,000 x g.

Temperature control

This model is typically a non-refrigerated benchtop centrifuge.

Footprint

Compact designs are typical, often around 24 cm (W) x 35 cm (D) x 22 cm (H).

Noise level

Units of this specification commonly operate below 56 dB(A).

Power supply

Typically operates on standard laboratory mains power, commonly 230V, 50/60Hz for the UK market.

• What types of samples can this centrifuge process?

  This centrifuge is designed for micro-volume samples in molecular biology, such as DNA/RNA, proteins, and cell pellets, typically in 0.2 mL to 2.0 mL tubes.

• Is this unit suitable for temperature-sensitive samples?

• What maintenance is typically required for a centrifuge of this type?

• Can this centrifuge accommodate different tube sizes?

• What safety features are typically included?

Centrifuges are valued across laboratories and biotech facilities for reliable sample separation. Buyers typically prioritise rotor compatibility, temperature stability in refrigerated models, and ease of maintenance when purchasing used units. Industry sentiment reflects appreciation for durable construction and consistent performance. Key considerations include verifying motor condition, checking calibration history, and confirming availability of compatible rotors. Well-maintained examples offer strong value and longevity, though wear on seals and bearings warrants inspection. Speed range and capacity alignment with intended applications remains important for operational efficiency.

General overview for this equipment type